Sheep Alkaline Phosphatase, Tissue-nonspecific Isozyme(ALPL) ELISA Kit

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Product details
Product Name: Sheep Alkaline phosphatase, tissue-nonspecific isozyme(ALPL) ELISA kit
Product Type : ELISA Kit
Code : CSB-EL001631SH
Size : 96T,5×96T,10×96T
Uniprot NO. : W5PFB9
Abbreviation : ALPL
Alias : AP-TNAP, FLJ40094, FLJ93059, HOPS, MGC161443, MGC167935, TNAP, TNSALP, OTTHUMP00000002972|alkaline phosphatase, tissue-nonspecific isozyme|alkaline phosphomonoesterase|glycerophosphatase|liver/bone/
Species : Sheep
Sample Types : serum, plasma, tissue homogenates
Detect Range : 15.6 mU/ml-1000 mU/ml
Sensitivity : 3.9 mU/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 7-14 working days
Research Area : Immunology
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Target details : There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.
HGNC : 438
RGD : 2100
MGI : 87983
Precision :

Intra-assay Precision (Precision within an assay): CV%<8

%Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity :

To assess the linearity of the assay, samples were spiked with high concentrations of sheep ALPL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

             Sample             Serum(n=4)

1:1         Average %               102

             Range %              97-106

1:2         Average %                95

             Range %                91-99

1:4         Average %                92

             Range %                85-96

1:8         Average %                 93

             Range %                86-97

Recovery :

The recovery of sheep ALPL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type            Average % Recovery       Range

Serum (n=5)              92                            87-96

EDTA plasma (n=4)        104                          97-108

Typical Data :

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml      OD1     OD2     Average     Corrected

1000     1.945    1.854      1.900        1.749

500      1.476    1.427      1.452        1.301

250      1.124    1.074      1.099        0.948

125      0.776    0.784      0.780        0.629

62.5     0.573    0.581      0.577        0.426

31.2     0.357    0.361      0.359        0.208

15.6     0.248    0.245      0.247        0.096

0          0.152    0.150      0.151

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